The Virginia Opossum (Didelphis virginiana) is the only marsupial found in North America north of the Rio Grande River. A solitary and nocturnal animal about the size of a domestic cat, it is a successful opportunist and is found throughout North America from coast to coast (introduced to California in 1910), and from Central America and Mexico to southern Canada; it seems to be still expanding its range northward. Its ancestors evolved in South America, but were enabled to invade North America in the Great American Interchange by the formation of the Isthmus of Panama about 3 million years ago. It is often seen near towns, rummaging through garbage cans, or lying by the side of the road, a victim of traffic.

The Virginia Opossum is the original animal named "opossum". The word comes from Algonquian wapathemwa, not Greek or Latin, so the plural is opossums. Colloquially, the Virginia Opossum is frequently called simply possum. The name is applied more generally to any of the other marsupials of the Didelphimorphia and Paucituberculata orders, which includes a number of opossum species in South America.